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. 2018 May 8;13(5):e0196840. doi: 10.1371/journal.pone.0196840

Fig 3. Resistance to HAP and 5-FU conferred by overexpression of APT2 and APT1.

Fig 3

The cloned APT2 plasmid and a PCR-amplified APT1 gene cloned into the vector pHR81 were tested for resistance to HAP and 5-FU. Cells transformed with pHR81 [30] were included as a negative control. Transformants were grown in liquid medium to late exponential phase, serially diluted, and then spotted onto uracil- and adenine-less SC galactose (Gal-UA) plates with or without HAP or 5-FU at the indicated concentrations.