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. 2018 May 8;13(5):e0196840. doi: 10.1371/journal.pone.0196840

Fig 5. Sensitivity to HAP and 5-FU due to disruption of different resistance genes.

Fig 5

Yeast strains disrupted for the genes isolated in our HAP resistance screen and in our previous screen for 5-FU resistance [16] were tested for increased sensitivity to HAP (A) and 5-FU (B and C). All strains were transformed with the empty vector pHR81 in order to complement the ura3 mutation in the genetic background and thus restore a functional pyrimidine biosynthesis. The + sign stands for the haploid wild type control strain BY4742, +/+ stands for the diploid wild type control strain BY4743, and +/dut1 for the diploid DUT1/dut1 heterozygote. Transformants were grown in liquid medium to late exponential phase, serially diluted, and then spotted onto uracil- and adenine-less SC galactose plates (Gal-UA) plates, uracil-less glucose (Glu-U) or uracil-less galactose (Gal-U) plates with or without HAP or 5-FU at the indicated concentrations. The no drug control plates were photographed after 3 days and the drug plates after 5 days.