Fig 2. Percentage growth at 100 μM (panel A) and IC50 (panel B) of selected compounds.
A. The percentage growth at 100 μM was determined by the XTT assay. Percentage growth was calculated using the following formula: (Esample-Enc)/(Egc-Enc)*100%. Percentage growth for each compound in the Pathogen Box is indicated with a red dot, and in the Stasis Box with a blue dot. Compounds with a growth reduction of more than 80% and thus a growth below 20% (black line) were considered compounds able to inhibit M. mycetomatis growth. Of these compounds the IC50 was determined. B. The IC50 was defined as the concentration of compound in μM were 50% reduction in fungal growth was obtained. The obtained IC50 for the 215 compounds analysed were indicated with a red dot (Pathogen Box) or a blue dot (Stasis Box). Compounds with IC50s below 5 μM (black line) were the most potent compounds which were analysed further.