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. 2018 Apr 26;12(4):e0006437. doi: 10.1371/journal.pntd.0006437

Fig 7. Fungal burden in G. mellonella larvae infected with M. mycetomatis and treated with selected compounds.

Fig 7

In panels A, B and C, histopatholocial sections of larvae are demonstrated which were treated with the different compounds and sacrificed 72h after inoculation. Histophatological sections are stained with Grocott to demonstrate the presence of fungal grains (black stained) and indicated by arrows. Panel A, demonstrate a larvae treated with PBS as a control, for which both large grains (L) and smaller grains (S) are visible. Panels B and C show G. mellonella infected with M. mycetomatis and treated with MMV688942 (Bitertanol)(B), MMV689244 (EPL-BS1246)(C). The scale bar present on each image represents 500μm. By counting the grains on these histological sections of 3–5 larvae per group, the number of grains (panel D) and the total grain size (panel E) per larvae per treated compound or PBS (control group) was determined. Melanisation of the larvae was determined by measuring the OD405nm of the hemolymph in all groups (panel F). PBS in all panels corresponds to M. mycetomatis infected larvae, treated with PBS. This is the control group. Significant differences determined using the Mann-Whitney U-test were displayed as * (0.01<p<0.05), ** (0.001<p<0.01), or *** (p<0.001).