Table 1. Mean open durations for different agonists after oxidation and reduction of αD97C/αY127C receptors.
| Agonist/receptor (number of transfections) | Treatment (number of patches) | mOpen1 | Area1 | mOpen2 | Area2 |
|---|---|---|---|---|---|
| ACh/αD97C/αY127C (8) | Control (4) | 2.3 ± 0.3 × 10−4 | 0.4 ± 0.08 | 8.0 ± 0.8 × 10−4 | 0.7 ± 0.10 |
| H2O2 (12) | 2.0 ± 0.1 × 10−4 | 1.0 ± 0.03 | |||
| DTT (8) | 3.0 ± 0.1 × 10−4 | 0.6 ± 0.04 | 8.5 ± 0.1 × 10−4 | 0.4 ± 0.06 | |
| ACh/wild type (3) | Control (4) | 1.9 ± 0.1 × 10−5 | 0.2 ± 0.05 | 1.2 ± 0.1 × 10−3 | 0.8 ± 0.08 |
| H2O2 (8) | 2.0 ± 0.2 × 10−5 | 0.1 ± 0.01 | 1.3 ± 0.1 × 10−3 | 0.9 ± 0.09 | |
| DTT (6) | 1.8 ± 0.1 × 10−5 | 0.2 ± 0.04 | 9.4 ± 0.7 × 10−4 | 0.7 ± 0.06 | |
| SubCho/αD97C/αY127C (5) | Control (6) | 6.5 ± 0.5 × 10−4 | 1.0 ± 0.02 | ||
| H2O2 (12) | 6.9 ± 0.1 × 10−5 | 0.2 ± 0.04 | 1.8 ± 0.5 × 10−4 | 0.8 ± 0.06 | |
| DTT (8) | 5.4 ± 0.6 × 10−4 | 1.0 ± 0.02 | |||
| SubCho/wild type (3) | Control (4) | 2.6 ± 0.14 × 10−5 | 0.3 ± 0.05 | 2.9 ± 0.2 × 10−4 | 0.7 ± 0.08 |
| H2O2 (4) | 1.9 ± 0.11 × 10−5 | 0.4 ± 0.06 | 2.8 ± 0.1 × 10−4 | 0.6 ± 0.09 | |
| DTT (4) | 2.1 ± 0.14 × 10−5 | 0.3 ± 0.05 | 2.7 ± 0.1 × 10−4 | 0.7 ± 0.10 | |
| CCh/αD97C/αY127C (3) | Control (4) | 7.3 ± 0.2 × 10−5 | 0.8 ± 0.07 | 1.8 ± 0.8 × 10−4 | 0.3 ± 0.07 |
| H2O2 (6) | 6.0 ± 0.9 × 10−5 | 0.7 ± 0.06 | 2.8 ± 0.1 × 10−4 | 0.3 ± 0.04 | |
| DTT (8) | 4.9 ± 0.5 × 10−5 | 0.7 ± 0.06 | 2.3 ± 0.2 × 10−4 | 0.3 ± 0.05 | |
| CCh/wild type (3) | Control (4) | 3.2 ± 0.3 × 10−4 | 1.0 ± 0.03 | ||
| H2O2 (6) | 3.1 ± 0.3 × 10−4 | 1.0 ± 0.02 | |||
| DTT (4) | 2.9 ± 0.3 × 10−4 | 1.0 ± 0.03 | |||
| PIP/αD97C/αY127C (3) | Control (4) | 2.1 ± 0.1 × 10−4 | 1.0 ± 0.03 | ||
| H2O2 (6) | 2.1 ± 0.5 × 10−4 | 1.0 ± 0.02 | |||
| DTT (6) | 2.2 ± 0.7 × 10−4 | 1.0 ± 0.05 | |||
| PIP/wild type (3) | Control (4) | 2.5 ± 0.2 × 10−4 | 1.0 ± 0.04 | ||
| H2O2 (4) | 2.9 ± 0.2 × 10−4 | 1.0 ± 0.03 | |||
| DTT (4) | 2.9 ± 0.2 × 10−4 | 1.0 ± 0.03 | |||
| TMA/αD97C/αY127C (3) | Control (4) | 3.8 ± 1.0 × 10−4 | 1.0 ± 0.02 | ||
| H2O2 (6) | 4.1 ± 0.3 × 10−4 | 1.0 ± 0.03 | |||
| DTT (4) | 3.8 ± 0.5 × 10−4 | 1.0 ± 0.03 | |||
| TMA/wild type (3) | Control (4) | 3.9 ± 0.4 × 10−4 | 1.0 ± 0.03 | ||
| H2O2 (4) | 4.1 ± 0.2 × 10−4 | 1.0 ± 0.03 | |||
| DTT (4) | 3.9 ± 0.3 × 10−4 | 1.0 ± 0.05 | |||
| Cho/αD97C/αY127C (4) | Control (4) | 2.1 ± 0.2 × 10−4 | 1.0 ± 0.03 | ||
| H2O2 (8) | 2.0 ± 0.3 × 10−4 | 1.0 ± 0.03 | |||
| DTT (6) | 2.2 ± 0.7 × 10−4 | 1.0 ± 0.04 |
For each agonist/receptor/treatment combination, mean open durations and relative areas from a fit of the sum of exponentials were determined. Values listed are the mean ± SD for the indicated number of patches.