Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2018 May 7;217(5):1719–1738. doi: 10.1083/jcb.201604108

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2018 Fassier et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms/). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 International license, as described at https://creativecommons.org/licenses/by-nc-sa/4.0/).

PMC Copyright notice

Figure 2. — Fignl1 associates with MTs in spinal motor axons. (A) Immunodetection of Fignl1 (red), acetylated α-tubulin (blue), and GFP (green) in 26-hpf Tg(Hb9:GFP) transgenic embryos. Lateral views of the trunk. (B) GC from cultured Tg(Hb9:GFP) SMN labeled with phalloidin (green), tyrosinated tubulin (blue), and Fignl1 (red) antibodies. Arrows indicate actin protrusions. (C) Detergent extraction of soluble proteins from WT or Tg(Hb9:GFP) spinal neurons immunostained by tyrosinated tubulin (red) and Fignl1 or GFP (green). Bars: (A and B) 10 µm; (C) 20 µm. (D) MT copelleting assay from COS-7 cells transfected with pCS2⁺-HA or pCS2⁺-HA-Fignl1 (HA-Fignl1). Supernatants (S) were incubated with taxol and GTP to induce MT polymerization and centrifuged on a sucrose cushion to pellet MTs and their associated proteins (P2). Soluble tubulins and proteins that did not bind MTs remained in the S2 supernatant. WBs assessed Fignl1 and α-tubulin levels in different fractions. L, total lysate.