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. 2018 May 7;217(5):1757–1776. doi: 10.1083/jcb.201708168

Figure 2.

Figure 2.

Prolonged and strong inhibition of the proteasome causes cells to induce the mRNAs for nearly all Atg genes and Ub receptors. (A) When treated with 100 nM BTZ for 20 h, SH-SY5Y cells induced the mRNAs for nearly all Atg genes and Ub receptors but less than did p62 and GABARAPL1. (B) SH-SY5Y cells induced the mRNAs of most Atg genes when treated with 100 nM BTZ for 20 h (right). Reducing the BTZ concentration to 10 nM (right) or the treatment time to 13 h (left) prevented induction. (C and D) MM1.S cells induced the mRNAs of most Atg genes when treated with 20 or 50 nM BTZ for 19 h, although much less than GABARAPL1 and p62 (C). Reducing the BTZ concentration to 10 nM (C) or the treatment time to 14 h (D) failed to induce most genes. In this figure and Fig. 3, the exact fold increase is shown for p62 and GABARAPL1 because their mRNAs increase >10-fold. *, P < 0.05. Error bars indicate SD.