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. 2018 May 7;217(5):1797–1813. doi: 10.1083/jcb.201710095

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Figure 7. — Molecular interaction between ORP5 and ORP8 affects their PM localization. (A) Representative live-cell confocal images of cells expressing full-length ORP5 and ORP8 (left) and the cartoon of their structural features (right). Abbreviations are the same as used in Fig. 1, and the yellow box designates the acidic N-terminal stretch present in ORP8 but not ORP5. HEK293-AT1 cells were cotransfected with mCherry-tagged ORP5 and GFP-tagged ORP8 for 1 d for confocal analysis. Note the lack of punctate ORP5 signal in the ORP8-expressing cell. (B) Representative images showing cells coexpressing GFP-ORP5 and mCherry-tagged ORP8 in which the PH domain was replaced by an FKPB12 module (mCherry-FK-ORP8, purple box). mCherry-FK-ORP8 expressing cells show more ORP5 showing up in the ER, but still preserve some PM puncta. (C) Representative images showing cells coexpressing full-length GFP-ORP5 and mCherry-tagged ORP8 N-terminally truncated to the same point defined as the start of ePH as described in Fig. 3. Note that the truncated ORP8 is less effective in preventing ORP5 PM localization and shows punctate localization itself. Bars, 10 µm.