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. 2018 May 7;217(5):1613–1622. doi: 10.1083/jcb.201801044

Figure 4.

Figure 4.

Basal mitophagy is minimally affected in Pink1 mutants. Confocal microscopy analysis of mito-QC reporter in Pink1B9 mutant larval epidermis and CNS, adult brain (2 d old), DA neurons at 2 and 30 d old, and adult flight muscle (2 d old), as indicated. Mitolysosomes are evident as GFP-negative/mCherry-positive (red-only) puncta. Bars, 10 µm. Charts show quantification of mitolysosomes and show mean ± SD of n = 6 animals. Number of cells analyzed: epidermis, WT = 29 cells, Pink1B9 = 29 cells; larval CNS, WT = 28 cells, Pink1B9 = 26 cells; adult brain, WT = 74 cells, Pink1B9 = 66 cells; DA neurons 2 d, WT = 100 cells, Pink1B9 = 54 cells and 30 d, WT = 78 cells, Pink1B9 = 82 cells. Quantification of WT control samples is the same as shown in Figs. 1 and 5. Statistical significance determined by Welch’s t test; ns, nonsignificant. Genotypes analyzed were PinkB9/Y;da-GAL4/UAS-mito-QC (epidermis and larval CNS), PinkB9/Y;UAS-mito-QC/+;nSyb-GAL4/+ (adult brain), PinkB9/Y;UAS-mito-QC/+;TH-GAL4/+ (DA neurons), and PinkB9/Y;Mef2-GAL4/UAS-mito-QC (adult muscle).