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. Author manuscript; available in PMC: 2018 Aug 16.
Published in final edited form as: Oncogene. 2018 Feb 16;37(18):2422–2431. doi: 10.1038/s41388-018-0134-2

Figure 1. USP9X binds and stabilizes YAP1.

Figure 1

(A–B) HEK293T cell were lysed and IPed with indicated antibodies. The immunocomplexes were subjected to Western blot. (C) MDA-MB-231 Cells stably expressed ctrl or USP9X shRNAs were subjected to western blot and qRT-PCR to examine the indicated protein and mRNA level. Lower panel: YAP1 mRNA level relative to β-actin was quantified (mean ± s.d. (n=6)). (D) Cells as in (C) were left untreated or treated with MG-132 and Western blot was performed to examine the indicated protein levels. (E) CHX pulse-chase assay was performed in cells as in (C). Right panel: the protein levels of YAP1 relative to β-actin (mean ± s.e.m. of three independent experiments)