Video 1. Single-molecule TIRF microscopy of TS-NDC80 modules on dynamic microtubules.

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DOI: 10.7554/eLife.36764.015

35 pM of T₀S₄[NDC80^TMR]₄ (green) in the presence of 8 µM tubulin labelled with HiLyte-642 (red) and in the absence of KCl. The two-channel images were acquired every 1.1 s (shown at 30 fps). Top left corner shows time in min:sec. White arrows mark tip-tracking events. Scale bar 5 µm.