Video 3. A microtubule is rescued five times at the bead attachment site.

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DOI: 10.7554/eLife.36764.018

A bead coated with 0.3% PLL-PEG-biotin and then saturated with T₁S₃[NDC80^FAM]₃ was attached to a microtubule with a trap. The microtubule experiences dynamic instability, but its shortening is five times in a row rescued at the attached bead (at 2, 9, 24, 47 and 60 min from the start of the experiment, see timer in the top left corner). Note that each rescue is preceded by a displacement of the bead along the microtubule axis in the direction of disassembly. After 63 min, the trap stiffness is increased from initial 0.03 pN/nm to 0.13 pN/nm to manually remove the bead from the microtubule. The microtubule without the attached bead depolymerizes without stalling or rescue events. Scale bar 5 µm.