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. 2018 Apr 20;9(30):20993–21006. doi: 10.18632/oncotarget.24772

Figure 2. Kinetic study of cellular internalization of S15-APT QDs into A549 cells.

Figure 2

A549 cells were incubated with 50 nM S15-APT QDs for 10 min, 0.5 h, 1 h, 2 h, 4 h, and 6 h at 37° C. Nuclei were stained with 2 μg/ml Hoechst 33342. Fluorescence confocal microscopy was performed using an inverted confocal microscope (Zeiss LSM 710) at ×630 magnification. Quantification of the average number of endolysosomes per cell was determined using Imaris Software. The red fluorescence channel was defined between 10-100 for all presented images. Fitting the dependence of the average number of fluoresent endolysosomes/cell (Y(t)) to the incubation time (t) was performed by nonlinear curve fitting (Eq. 1).