Figure 2. Impact of celastrol on frequency of necrotic (A) and apoptotic (B) cell in LOVO/DX cell cultures.

The cells (5×10⁵/ml) were incubated [4h, 37°C] in the absence (control) or presence of celastrol, and then double stained with Annexin V-FITC and PI fluorescent probes (FITC Annexin V Apoptosis Detection Kit I BD Biosciences). The result are presented as a percentage of dead cells (PI⁺), early apoptotic (EA) cells (Annexin V-FITC⁺ and PI⁻) and late apoptotic (LA) cells (Annexin V-FITC⁺ and PI⁺). The dose-dependent effects were calculated with regression equations (mean±SD; n=4).