Table 1. Effect of celastrol on ALDH1 fluorescence in LOVO/DX cells with ALDEFLUOR™.
ALDEFLUOR™ (Median Fluorescence Intensity) |
|
---|---|
Control cells | 20,58 |
Celastrol [1μM] | 18,31 |
Celastrol [10μM] | 16,14 |
LOVO/DX cells were pre-treated with celastrol for 30 minutes and then incubated with Hoechst33342 for 90 minutes. Following incubation, cells were stained with ALDEFLUOR™ and analyzed by flow cytometry