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. 2018 Apr 20;9(30):20953–20964. doi: 10.18632/oncotarget.25104

Figure 3. DHX9 and ribosomal proteins are associated with viral circular RNA.

Figure 3

(A) HepAD38 cells with or without HBV expression (On or Off) were used for DHX9 precipitation. Normal rabbit IgG was used for the negative control (NC). DHX9 precipitation was confirmed by western blotting. A representative image of two independent experiments is shown. (B) RNAs were extracted from the immunoprecipitations and reverse transcribed using random primers. PCR was performed using primer set #2. RNAs that were not reverse transcribed were included as negative controls (RT(−)). Mr, DNA marker. A representative image of two independent experiments is shown. (C) Ribosomal P0/P1/P2 protein (Ribo0-2) was immunoprecipitated as described in (a). Five percent of the cell lysates was used as an input. A representative image of two independent experiments is shown. (D) Extracted RNAs from the immunoprecipitations (RIP) were treated with or without RNase R, followed by reverse transcription using random primers. PCR was performed using primer set #2 and MCl1 gene primers as a control. RNA extracted directly from cells was used as an input. RNAs that were not reverse transcribed were included as negative controls (RT(−)). Mr, DNA marker. A representative image of two independent experiments is shown.