Table 1. The mutant-acquired PTEN−/− characteristics reversed by overexpression TPTE2.
Characteristic | Relative levelb | ||
---|---|---|---|
MCF-10A | PTEN−/− | PTEN−/− TPTE2oe | |
Wound healing, +GFsa | +++ | ++++ | ++ |
Wound healing, +S, –other GFsa | ++ | ++++ | + |
Wound healing, –GFsa Division rate, +GFsa |
– ++ |
++++ ++++ |
– ++ |
Viability, on 2D substrate, –GFsa, undisturbed |
– | ++++ | – |
Adhesion on 2D substrate and long term viability, –GFsa |
– | ++++ | – |
Viability in 3D matrix, –GFsa |
– | ++++ | – |
Expression of annexin V surface binding site, +GFsa |
+++ | – | ++++ |
a +GFs: plus growth factors. +S, –other GFs: plus serum, minus growth factors.
b “Relative level” represents a comparison to the maximum measured amongst the three genotypes. Maximum is ++++, descending (+++, ++, +) to zero (−).