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. 2018 Apr 20;9(30):21141–21155. doi: 10.18632/oncotarget.24975

Figure 3. HER2 expression remains unchanged after chronic exposure to T-DM1.

Figure 3

(A) mRNA and (B) proteinexpression from total cell lysates show that HER2 levels are unaffected in resistant cells. (C) HER2 expression at the cell surface determined by flow cytometry shows that parental and resistant cells express the same amount of HER2. (D) After exposure to T-DM1 for 1h at 4°C, cells were stained with anti-Kappa antibody and the mean fluorescence intensity (MFI) was studied using flow cytometry. T-DM1 was found to bind similarly parental and resistant cells. A single experiment is shown, representative of 3 experiments.