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. 2018 Apr 20;9(30):21141–21155. doi: 10.18632/oncotarget.24975

Figure 4. T-DM1-induced cell cycle arrest is impaired in OE-19 TR and TCR compared to OE-19 S.

Figure 4

(A) Exposure to increasing concentrations of T-DM1 for 24h and analysis of cell cycle distribution shows that the G2/M population was decreased in resistant cells compared to parental. Statistically differences are shown for TR (***: P<0,001; *: P<0,05) and TCR (+++: P<0,001) compared to S. (B) Cell cycle distribution was studied by propidium iodide staining using flow cytometry after 24h exposure to 1 μM vincristine or 10 nM S-methyl DM1. Control conditions are not shown; only OE-19 S, TR and TCR exposed to drugs are plotted. Vincristine and S-methyl DM1 induced G2/M phase arrest in parental and resistant cells. The TCR cell line showed decreased sensitivity to cell cycle arrest in comparison to the parental cell line (*: P<0,05; **: P<0,01).