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. 2018 Apr 20;9(30):21141–21155. doi: 10.18632/oncotarget.24975

Figure 6. Morphology is modified in both resistant cell lines but only TCR cells have increased migration speed and decreased adhesion strength.

Figure 6

(A) Immunofluorescence staining of α-tubulin (green) and DAPI (blue) observed by confocal microscopy shows morphological differences between parental and T-DM1-resistant cell lines. (B) Migration speed determined by wound healing assay shows an increase in TR and TCR cells compared to parental (C) Immunofluorescence staining of talin (green) and DAPI (blue) observed using a confocal microscope. Focal adhesions in parental cells appear bigger and in least amount that those in resistant cells. (D) Detached fraction of parental and resistant cells following detachment by centrifugal force shows a decreased adhesion strength in TCR cells (*: P<0,05; P<0,01). (E) Cell death was quantified by Annexin/PI staining following 24h and 48h of incubation in suspension conditions. The percent of living cells was decreased in TR cells at 24h (P<0,01) and in TCR cells after 24h (P<0,001) and 48h (P<0,01) compared to parental. (F) Sensitivity to rhosin, bortezomib and fasudil was studied using MTT cytotoxic assays after 6 days exposure to the corresponding cytotoxic agents. Sensitivty to rhosin was increased in TCR cells compared to parental (**:P<0,01).