Table 1. Percentage of total apoptotic cells in Jurkat cell line and in T-ALL patients’ lymphoblasts after JWH-133 and RTX treatments.
| Jurkat | 6 h (%) | 12 h(%) | 24 h(%) |
|---|---|---|---|
| NT | 22.8 ± 2.3 | 15.1 ± 2.4 | 26.5 ± 2.7 |
| JWH-133 [100 nM] | 46.9 ± 2.3* | 28.5 ± 2.4* | 33.3 ± 2.5* |
| RTX [5 μM] | 52.3 ± 2.2* | 42.5 ± 2.4* | 30.8 ± 2.7 |
| ALL T PTS | 6 h (%) | 12 h (%) | 24 h (%) |
| NT | 13.8 ± 4.1 | 20.9 ± 4.9 | 14.4 ± 4.8 |
| JWH-133 [100 nM] | 32.9 ± 5.4* | 30.4 ± 3.1* | 26.8 ± 4.9* |
| RTX [5 μM] | 30.2 ± 4.2* | 32.5 ± 4.7* | 26.5 ± 4.8* |
Analysis of cell apoptosis by Annexin-V and PI double-stained in Jurkat cells line and T-ALL patients’ lymphoblasts derived from 4 patients induced by JWH-133 [100 nM] and RTX [5 uM], after 6 h, 12 h and 24 h of exposure. The results are presented as the mean percentage ± SD of three independent experiments for Jurkat cells and as mean percentage ± SD of 4 single experiments on each one of the 4 patients. The results were analyzed by one-way analysis of variance (ANOVA). *indicates p ≤ 0.05 compared to the untreated control (NT).