PG activity accumulates in conjunction with germination. A, The activity of PG from desiccated developing and mature seeds and mature seeds during germination and subsequent radicle protrusion as assessed using a reducing sugar assay in the presence of 5 mm CaCl2. From 48 h onward seeds were separated into those that had and had not completed germination. IG, Immature green; MG, mature green; B, breaker; M, mature. B, The effect of 5 mm CaCl2 on the activity of PG from whole cv Moneymaker seeds allowed to imbibe for 48 h from which the radicle had protruded. Activity is reported as picomoles of GalUA produced per hour per seed.