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Southern-blot analysis of tomato genomic DNA probed with 1,504 bp of the poly(A)-truncated PG washed at low (5× SSC and 0.1% SDS at 65°C; A) and high (0.2× SSC and 0.1% SDS, 65°C; B) stringency suggest that there is little homology among the PG transcript and other members of this large gene family in tomato. The number of bands visible on the blot corresponds to the number predicted based on the restriction map of the cDNA (accession no. AF138858) and genomic clone (not shown).
