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. 2018 Apr 20;9(30):21166–21181. doi: 10.18632/oncotarget.24989

Figure 2. Lymphoma cells prefer oxidative phosphorylation over glycolysis.

Figure 2

Daudi cells (A, B) and SUDHL-4 (C, D) were pretreated with 0 μM (black), 100 μM (white), 1 mM (grey) of metformin for 7 days and their metabolic state was determined using the Seahorse XF Cell Energy Phenotype kit (Agilent) in triplicate. In short, after basal Oxygen Consumption Rate (OCR) and Extracellular Acidification Rate (ECAR) measurements, cells were stressed with the combination of 1 μM oligomycin and 0.5 μM FCCP for Daudi or 1 μM FCCP for SUDHL-4 and stressed OCR and ECAR measurements were taken. No metformin was present during the assay (2 hours). (A, C) OCR: The rate of decrease of oxygen concentration in the assay medium. OCR is a measure of the rate of mitochondrial respiration of the cells. (B, D) ECAR: The rate of increase in proton concentration [or decrease in pH] in the assay medium. ECAR is a measure of the rate of glycolysis of the cells. Baseline: OCR and ECAR of cells at starting assay conditions (specifically, in the presence of non-limiting quantity of substrates). Stressed: OCR and ECAR of cells under an induced energy demand (specifically, in the presence of stressor compounds). p values for basal OCR/ECAR at 0 μM vs 1 mM of metformin and basal OCR/ECAR vs. stressed OCR/ECAR at 1 mM metformin is shown. *****p value < 0.0001.