Table 2.
Antibodies used in this study.
| Antibody | Source | Catalogue # | Concentration | Reacts with: |
|---|---|---|---|---|
| α-actin | Sigma | A2547 | 1/2000 | Smooth muscle |
| Estrogen receptor alpha (ESR1) | Abcam | Ab16660 | 1/100 | Estrogen receptor |
| HOXA11 | Sigma | HPA035623 | 1/400 | Uterine mesenchyme |
| ISL1 | Abcam | Ab20670 | 1/200 | Vaginal mesenchyme |
| Keratin 6 | Acris Antibodies | AM21068PU-S | 1/200 | Basal cells of stratified epithelia |
| Keratin 7 | E.B. Lanea | LP1K | 1/10 | Simple columnar (glandular) epithelia |
| Keratin 8 | E.B. Lanea | LE41 | 1/10 | Simple columnar (glandular) epithelia |
| Keratin 10 | Dako | M7002 | 1/50 | Terminally differentiated squamous cells |
| Keratin 14 | BioGenex | LL002 | 1/100 | Basal cells of stratified epithelia |
| Keratin 19 | E.B. Lanea | LP2K | 1/10 | Simple columnar (glandular) epithelia |
| TP63 | Santa Cruz Biotechnology | sc-8343 | 1/100 | Transcription factor |
| Progesterone receptor | Abcam | Ab16661 | 1/100 | Progesterone receptor |
| Androgen receptor | Genetex | GTX62599 | 1/100 | Androgen receptor |
| Runx1-3 | Abcam | Ab92336 | 1/100 | Transcription factor |
| Uroplakin1 | T. T. Sunb | 1/100 | Urothelium |
a
Institute of Medical Biology, Singapore.
b
New York University, New York.