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. Author manuscript; available in PMC: 2019 May 20.
Published in final edited form as: Free Radic Biol Med. 2018 Mar 11;120:89–101. doi: 10.1016/j.freeradbiomed.2018.03.010

Figure 1.

Figure 1

Plasma 8-iso-PGF2α measurements. Plasma 8-iso-PGF2α levels were measured pre- and post-surgery using the OxiSelect™ 8-iso-prostoglandin F2α ELISA kit. A standard curve of 200,000 ng/mL, 50,000 ng/mL, 12,500 ng/mL, 3125 ng/mL, 781ng/mL, 195 ng/mL, and 49 ng/mL was analyzed by a “log inhibitor vs response, variable slope with 4-parameters” equation to determine 8-iso-PGF2α concentration. The animals that underwent LAD ligation/reperfusion (LAD 1, 2, and 3) had a significant increase (p value < 0.05) in 8-iso-PGF2α post-surgery as compared to pre-surgery. No significant increase in 8-iso-PGF2α levels was detected in the animals that underwent the sham surgery (Controls 1 - 3).