Table 1.
Behavioral characterization of decision-making mutant larvae.
| Mutant Allele | Relative Bias (26 dB) | Total Response % (26 dB) | Relative Bias (13 dB) | Spontaneous Activity (pixels/sec) | Acoustic Startle Habituation | Visual (Dark Flash) Response Latency (ms) |
Complementing Alleles |
|---|---|---|---|---|---|---|---|
|
wrong turn p190 (CaSR) |
−75±4 (N=58) | 93±1 (vs 98, p<10−2) | −57±18 (vs −41, p=0.54) | 55±5 (vs 51, p<0.97) | 68%±6 (vs 72%, p<0.66) | 366±23 (vs 330, p=0.16) | p191, p192, p196 |
|
fashionably late p191 |
−72±3 (N=69) | 77±3 (vs 96, p<10−7) | −83±10 (vs −58, p=0.09) | 20±1 (vs 27, p<10−5) | 56%±7 (vs 41%, p<0.06) | n.d. | p190, p192, p193 |
|
procrastinator p192 |
−30±4 (N=58) | 84±4 (vs 94, p<10−3) | −97±3 (vs −46, p<0.0001) | 30±1 (vs 27, p<0.10) | 70%±5 (vs 72%, p<0.74) | 198±7 (vs 188, p=0.29) | p190, p191 |
|
better late than never p193 |
−93±1 (N=65) | 67±4 (vs 77, p<0.11) | −57±8 (vs −52, p=0.75) | 37±1 (vs 39, p<0.26) | 94%±2 (vs 94%, p<0.95) | 270±7 (vs 246, p=0.019) | p191 |
|
snooze button p194 |
−40±4 (N=74) | 83±3 (vs 96±1, p<10−4) | −33±18 (vs −36, p=0.90) | 24±1 (vs 24, p<0.64) | 92%±3 (vs 79%, p<0.0005) | 244±23 (vs 327, p=0.0033) | p195 |
|
indecisive p195 |
−23±5 (N=88) | 90±2 (vs 98±1, p<10−5) | −70±11 (vs −54, p=0.27) | 22±1 (vs 23, p<0.68) | 86%±2 (vs 90%, p<0.12) | 255±18 (vs 269, p=0.51) | p194 |
|
late responder p196 |
−71±3 (N=59) | 82±3 (vs 95, p<10−3) | −98±2 (vs −45, p<0.0001) | 22±2 (vs 26, p<0.10) | 69%±4 (vs 71%, p<0.63) | 275±10 (vs 270, p=0.72) | p190 |
|
biased dp197 |
−49±5 (N=83) | 85±2 (vs 98, p<10−6) | −76±9 (vs 66, p<0.0001) | 36±1 (vs 31, p<0.011) | 87%±4 (vs 86%, p<0.92) | 201±6 (vs 189, p=0.22) | n.d. |
Mean±SEM values for each assay on 5–6 dpf larvae, significance by t-test (See STAR Methods).