Figure 6.

Qualitative effects of Ca²⁺ regulators on Adh/GUS expression patterns in seedlings. Five days after transfer to horizontal-positioned plates containing Ca²⁺ antagonists, seedlings were stained in X-Gluc solution for 16 h. Whole seedlings (A–E) and representative roots (F–J) for each treatment condition are shown. A and F, Seedling and root from vertical plates; B and G, horizontal plates without Ca²⁺ antagonists. Seedling and root from horizontal-positioned plates containing: C and H, 5 mm EGTA; D and I, 25 μm RR; and E and J, 0.5 mm gadolinium. Root tips of seedlings grown on horizontal-positioned plates containing 25 μm RR (K) and 0.5 mm gadolinium (L) are shown without GUS staining.