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. Author manuscript; available in PMC: 2019 May 15.
Published in final edited form as: J Immunol. 2018 Apr 4;200(10):3429–3437. doi: 10.4049/jimmunol.1701422

Figure 1. Generation of a mouse expressing an inducible human voltage-gated sodium channel, Scn5a.

Figure 1

(A) To create the Scn5a mice, a flox-stop-flox cassette from pCALSL-mir30 was inserted upstream of the human Scn5a-GFP cDNA, and this construct was targeted to the Rosa26 locus by electroporating into JM8.N4 C57BL/6N-derived ES cells. Two successfully targeted ES clones were injected into blastocysts and both lines were transmitted in the germline. (B) One line was selected to be bred to a CD4-Cre expressing strain, to yield Scn5a+CD4-Cre+ offspring.