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. Author manuscript; available in PMC: 2019 May 15.
Published in final edited form as: J Immunol. 2018 Apr 2;200(10):3556–3567. doi: 10.4049/jimmunol.1701504

Figure 3. Activation of JNK pathway is essential for CS-induced LTB4 and IL-1β production.

Figure 3

LPS-primed BMDMs (0.3 × 106 cells in 400 μl media) were stimulated with 100 μg/cm2 CS for 6 h in the presence or absence of MAPK inhibitors: AG-126 (10 μM), SB-202190 (10 μM) and BI-78D3 (10 μM). Zileuton was used as a positive control for inhibition of LTB4 synthesis. ELISA was performed to assess (a) LTB4 levels and (b) IL-1β levels in the BMDM cell culture supernatants. CS (100 μg/cm2) induced LTB4 levels were also measured in (c) mouse mast cells, mouse and human neutrophils in the presence of JNK inhibitor. Data representative of n=3. Data are expressed as mean ± SEM. **p< 0.01, ***p< 0.001 non-parametric t-test.