Skip to main content
. Author manuscript; available in PMC: 2019 May 1.
Published in final edited form as: J Mol Cell Cardiol. 2018 Mar 6;118:13–25. doi: 10.1016/j.yjmcc.2018.03.001

Figure 8. In contrast to acute stimulation, chronic β-adrenergic stimulation promotes HDAC5 nuclear export.

Figure 8

Cardiac myocytes were infected with adenovirus expressing GFP-tagged HDAC5 and stimulated with 10 μM Iso and/or 50 μM PE for 1 or 24 hrs. A and C. HDAC5-GFP was immunoprecipitated with a HDAC5 antibody and detected using a phospho-specific antibody for Ser-498/661 or a HDAC5 protein antibody. B and D. Cytosolic and nuclear extracts were analyzed by western blotting with HDAC5, lamin (nuclear marker), or GAPDH (cytosolic marker) antibodies. n = 3 for each panel. A and B: * vs. no drug; † vs. PE at 1 hr; ‡ vs. PE and Iso at 1 hr. C and D: * vs. no drug; † vs. Iso at 1 hr.