Table 1. Rat flow cytometry.
All data from flow cytometric analyses of blood and kidneys from Dahl SS rats drinking either 0.1M NaHCO3 (bicarbonate) or equimolar NaCl (vehicle) are presented in the table above. Data from 14 day high salt (HS) and 3 day only low salt (LS) fed animals are shown. All groups represent N=4-11 with absolute numbers for each group are presented in the table (left column). P is the result of unpaired Students t-test comparing vehicle and bicarbonate treated groups. For all analyses,
| 14 days HS | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| Blood | CD3 (% total Kidney) | CD4 (% of CD3) | CD44 (% of CD3) | CD69 (% of CD3) | FOXP3 (% of CD3/CD4) | IL-17 (% total Kidney) | M1 (% total Kidney) | M2 (% total Kidney) | IL-10 (% total Kidney) | TNFα (% total Kidney) | CD11b/c (% total Kidney) |
| Vehicle (n=4) | 54±1 | 55±2 | 19±2 | 1.2±0.3 | 2.5±0.3 | 8±1 | |||||
| Bicarbonate (n=5) | 57±2 | 53±2 | 14±2 | 0.9±0.3 | 4.4±0.6 | 6±1 | |||||
| P | N.S | N.S | N.S | N.S | 0.02* | N.S | |||||
| Kidney | |||||||||||
| Vehicle (n=10) | 2.1±0.3 | 57±1 | 47±4 | 1.8±0.4 | 1.7±0.3 | 1.1±0.2 | 0.26±0.03 | 0.12±0.02 | 2.8±0.3 | 3.2±0.4 | 0.29±0.04 |
| Bicarbonate (n=11) | 1.8±0.3 | 55±2 | 36±5 | 2.8±0.7 | 3.3±0.5 | 0.7±0.1 | 0.12±0.02 | 0.20±0.03 | 4.2±0.3 | 2.0±0.3 | 0.40±0.04 |
| p | N.S | N.S | N.S | N.S | 0.01* | N.S | 0.001** | 0.04* | 0.048* | 0.03* | N.S |
| 3 days LS | |||||||||||
| Blood | CD3 | CD4 | CD44 | CD69 | FOXP3 | IL-17 | M1 | M2 | IL-10 | TNFα | CD11b/c |
| Vehicle (n=5) | 57±1 | 54±1 | 15±2 | 2.4±0.7 | 4.8±0.8 | 5.4±0.5 | |||||
| Bicarbonate (n=5) | 57±2 | 55±1 | 18±1 | 1.6±0.5 | 5.8±0.7 | 5.6±1.1 | |||||
| P | N.S | N.S | N.S | N.S | N.S | N.S | |||||
| Kidney | |||||||||||
| Vehicle (n=5) | 1.1±0.2 | 59±1 | 58±1 | 0.9±0.1 | 1.6±0.2 | 1.3±0.5 | 0.30±0.06 | 0.20±0.05 | 3.0±0.5 | 2.4±0.5 | 0.22±0.06 |
| Bicarbonate (n=5) | 1.1±0.3 | 54±2 | 52±3 | 0.9±0.3 | 2.6±0.5 | 0.4±0.2 | 0.16±0.04 | 0.36±0.02 | 4.8±0.5 | 1.4±0.2 | 0.24±0.02 |
| p | N.S | 0.02* | N.S (0.07) | N.S | N.S | N.S | N.S (0.1) | 0.03* | 0.04* | N.S (0.11) | N.S |
| 14 days HS | |||||||||||
| Spleen | CD3 | CD4 | CD44 | CD69 | FOXP3 | IL-17 | M1 | M2 | IL-10 | TNFα | CD11b/c |
| Vehicle (n=5) | 15.8±0.8 | 57±1 | 40±2 | 5.2±0.7 | 4.2±0.2 | 4.4±0.5 | 8.6±0.7 | 6.4±0.5 | 5.8±0.8 | 5.8±0.8 | 6±0.4 |
| Bicarbonate (n=6) | 15.3±0.8 | 53±1 | 35±2 | 7.2±0.9 | 6±0.5 | 2.8±0.6 | 5.8±0.5 | 8.3±0.5 | 7.5±0.7 | 3±0.5 | 7.3±0.6 |
| P | N.S | N.S (0.06) | 0.04* | N.S | 0.015* | N.S (0.08) | 0.01* | 0.02* | N.S | 0.01* | N.S |
P<0.05 was considered significant.
Two studies were pooled for HS (HS) kidney analysis. In group 1 (n=5 + 5) blood and kidney samples were analyzed from Dahl SS rats fed a HS diet for 14 days that did not have prior surgery. In group 2 (n=5 + 6 for vehicle and bicarbonate, respectively) kidney and spleen were analyzed. Blood pressure (telemetry), urine analysis and histological measurements of injury were all performed in group 2 animals. CD3, IL-17, Il-10, M1, M2, TNFα and CD11b/c positive cells are all expressed as % of total kidney/spleen cells±SE. CD4, CD44 and CD69 positive cells are expressed as % of CD4 positive cells. FOXP3 positive cells are expressed as % of both CD3/CD4 positive cells.