Table 3. Antibodies and Reagents utilized.
| Antibody Name |
Company | catalog # | Lot # | Tube amount |
info | Antibody validation |
|---|---|---|---|---|---|---|
| CD3- PerCP | eBioscience | 46-0030-82 | E16590-104 | .5ml,100ug | anti-rat | Tipton A, et. al., Hypertension, 2014(60) |
| CD4-FITC, OX-35 | BD Bioscience | 554837 | 5190753 | .5ml,100ug | mouse α rat | Zimmerman MA, et. al., Am J Renal Physiology 2015 April(29) |
| FOX P3-APC | eBioscience | 17-5773-82 | E07303-1639 | .5ml,100ug | α-mouse/rat | Tipton A, et. al., Hypertension, 2014(60) |
| RORγ/RORC- PE | R&D Systems | IC6006P | ABHO0214091 | mouse IgG | Zimmerman MA, et. al., Am J Renal Physiology 2015 April(29) | |
| CD11b/c-PE, OX-42 | BD Pharmingen | 554862 | 1.0ml* | anti-rat | Dick AD., et. al., Br J Ophthalmol. 1995 Sep;79(9):834-40(61). | |
| CD11b/c-PE, OX-42 | eBioscience | 12-0110-80 | E15919-106 | 125ul/500ul | α rat | He j, et. al., Clin Cancer Res. 2009 Nov 15;15(22):6871-80(62). |
| CD206 PerCP/Cy 5.5 | Biolegend | 321122 | B175845 | .5ml | anti human | Kato M, et. al., J Immunol. 2007 Nov 1;179(9):6052-63(63). |
| F4/80-A488, EMRI | Bioss | bs-7058R-A488 | AE084243 | 100ul | rabbit | http://biossusa.com/store/datasheets/bs-7058R-A488 |
| IL-10-Alexa647, CC320 | Novus | NB100-63026AF6477 | 1007-081415 | 250ul | mouse ab | http://www.novusbio.com/IL-10-Antibody-CC320_NB100-63026AF647.html |
| CD44-FITC, OX-49 | BD Bioscience | 550974 | 4324539 | .2ml | mouse α rat | Zimmerman MA, et. al., Am J Renal Physiology 2015(29)& Tipton Am J PhysiolRegulIntegr Comp Physiol 2012(28) |
| CD69-PE, H1.2F3 | eBioscience | 12-0691-82 | E01332-1634 | .5ml | α-mouse | Yokoyama W, et. al., JImmunol, 1988, 141(2): 369-76(64). |
| TNFα-biotin | Bioss | bs-2081R-biotin | AE081731 | 100ul | rabbit | http://biossusa.com/store/bs-2081r-biotin.html |
| IL-17A-PE | eBioscience | 12-7177-81 | 4276914 | .25ml | α-mouse/rat | Baban B, et.al., PLoS One. 2015(65) 22;10(4):e0124059. |
| CD68-FITC | Bio-Rad | MCA341F | 1601 | 1.0 ml | mouse anti-rat monoclonal | Glorie,L.L. et al. Am J physiol Renal.2012.303:F681-8(66) |
| CD163-Alexa 647 | AbDSerotec | MCA342A647 | 913 | 1.0 ml | mouse anti-rat monoclonal | Park,E.S. et al. Vet Pathol 2014.51(6):1151-64(67) |
| IL-10-PE | BD Pharmingen | 555088 | 8021822 | 500 ul | mouse anti-rat monoclonal | http://bdbiosciences.com/pe, mouse anti-rat IL10-555088 |
| Streptavidin-APC | eBioscience | 17-4317-82 | E07261-1634 | .5ml | http://www.ebioscience.com/media/pdf/tds/17/17-4317.pdf | |
| FIX/Perm Buffer/FOXP3 fixation | eBioscience | NC9548464/00-5521-00 | E00027-1654 | Baban B, et. al., IntImmunol. 2005 Jul;17(7):909-19(68). | ||
| Collagenase IV | Sigma (St Louis, MO) | C5138-500mg | 500mg | Baban B, et. al., IntImmunol. 2005 Jul;17(7):909-19(68). | ||
| ACK Lysing Buffer | Quality Biological | 50983220 | 4 × 100ml | Baban B, et. al., IntImmunol. 2005 Jul;17(7):909-19(68). | ||
| CD11b-FITC | BD Biosciences | 78022 | B202614 | |||
| CD68-FITC | BD biosciences | 562117 | 2219733 | Kishimoto T, von dem Borne AEG, Goyert SM, et al., ed. Leucocyte Typing VI: White Cell Differentiation Antigens. London: Garland Publishing; 1997 | ||
| TNFα+PE | eBioscience | 12-7349-82 | E02153-1634 | Chirkova T, et. al. J Virology 2013 Dec;87(24):13466-79. doi: 10.1128/JVI.01741-13. Epub 2013 Oct 2. | ||
| CD206-APC | BioLegend | 321109 | B202690 | Le Cabec V, et al. 2005. J. Leukocyte Biol. 77:934. | ||
| IL-10-APC | BioLegend | 506806 | B244158 | Abrams J, et al. 1992. Immunol. Rev. 127:5 | ||
| Tyrosine Hydroxylase | abcam | Ab112 | GR286793-6 | 100ul (0.5mg/mL) | •Chen G et al.Antidyskinetic Front Neurosci11:112 (2017). | |
| Acetylcholine esterase | Invitrogen | PA5-21371 | SC2362081N | 100ul (1mg/mL) | ||
| PGP9.5 | Sigma | SAB4503057 | 310302 | 100ug |
All antibodies utilized in flow cytometry and immunohistochemical studies along with regents are listed in the table.