Fig. 1. Bulk analysis of [¹⁸F]HFB binding in MDA-MB-231 and Jurkat cells.

(a) Chemical structure and illustration of the presumed mechanism of binding of [¹⁸F]HFB. The lipophilic long chain ester is rapidly absorbed into the cellular membrane. (b) Analytical HPLC indicating >99% radiochemical purity. (c) Bulk analysis of [¹⁸F]HFB labelling and [¹⁸F]FDG uptake in two cell lines (Jurkat and MDA-MB-231), measured by gamma counting. Cells were incubated with 250 µCi/mL of [¹⁸F]FDG (n=5) or [¹⁸F]HFB (n = 13) at 37°C for 30 min. Data is presented as disintegrations per minute (dpm) per cell. Experiments were repeated ≥ twice. Error bars are Mean ± SEM, *p ≤ 0.05 and **p ≤ 0.01