VIC calcification in response to elevated glucose. Baboon VICs were cultured normal media (NM) and in calcification media (CM) for 5 and 7 days. Cultures were also challenged with low, 5.6 mM, glucose (Low Glucose) or high, 25 mM, glucose (High Glucose). Calcium deposition was measured with a colorimetric method. Data are normally distributed. Data were analyzed by two-way ANOVA with Bonferroni’s test, Mean ± S.E.M. of triplicate experiments.