Table I.
Stage and Treatment | Ecotype/Mutant
|
|||||||
---|---|---|---|---|---|---|---|---|
Ler | LER | Col-1 er2 | Col-1 | Ws-O | spy-4 | gaia | ga5-1 | |
mm | ||||||||
Anthesis pistils | 2.8 ± 0.2 | 3.2 ± 0.2 | 2.9 ± 0.3 | 3.2 ± 0.4 | 3.2 ± 0.2 | 2.8 ± 0.3 | 2.9 ± 0.3 | 3.2 ± 0.3 |
Emasculated without pollination | 4.1 ± 0.4b | 4.8 ± 0.4a | 3.7 ± 0.2c | 4.1 ± 0.3b | 4.5 ± 0.1ab | 4.9 ± 0.4a | 4.5 ± 0.4a | 5.0 ± 0.2a |
Emasculated with pollination | 11.5 ± 1.0c (6.7) | 16.7 ± 1.5a (8.3) | 11.3 ± 0.6c (10.6) | 16.0 ± 1.7a (15.3) | 17.3 ± 1.1a (10.6) | 14.6 ± 0.8b (5.7) | 9.5 ± 1.2d (4.1) | 10.5 ± 1.0cd (4.2) |
IAA, 10 μmol pistil−1 | 7.5 ± 0.9 (3.7) | 7.9 ± 2.4 (2.9) | 6.9 ± 0.7 (5.0) | 5.2 ± 0.4 (2.3) | ||||
NAA, 10 μmol pistil−1 | 6.8 ± 0.8 (3.1) | 8.9 ± 3.5 (3.6) | 4.0 ± 1.7ns (1.4)b | 5.9 ± 1.4 (3.2) | 8.7 ± 1.4 (2.8) | 7.6 ± 1.2 (2.9) | ||
BA, 0.1 μmol pistil−1 | 5.9 ± 0.8 (2.4) | 7.9 ± 0.5 (2.9) | 5.3 ± 0.6 (3.0) | 4.5 ± 1.3ns (1.5)b | ||||
BA, 1 μmol pistil−1 | 5.7 ± 0.4 (2.2) | 6.2 ± 1.1 (1.9)b | 2.9 ± 0.3ns (0)b | 5.1 ± 0.7 (2.2) | 5.4 ± 1.1ns (1.3)b | 6.1 ± 0.2 (2.0) | ||
GA3, 0.1 μmol pistil−1 | 8.2 ± 0.8 (4.2) | 11.0 ± 3.2 (4.8) | 5.9 ± 0.9 (3.8) | 7.4 ± 0.4 (4.9) | ||||
GA3, 1 μmol pistil−1 | 9.5 ± 1.5 (5.2) | 12.2 ± 1.6 (5.6) | 7.6 ± 0.2 (6.0) | 9.2 ± 1.1 (7.2) | ||||
GA3, 10 μmol pistil−1 | 10.0 ± 1.1 (5.6) | 14.5 ± 1.9 (7.0) | 9.2 ± 0.5 (7.9) | 10.6 ± 1.4 (8.9) | 9.8 ± 0.8 (4.9) | 8.4 ± 1.1 (2.7) | 5.3 ± 0.7 (1.5)b | 10.0 ± 1.1 (3.9) |
Results are means ± sd. Numbers in parentheses represent the fold increase in silique length as described in “Results.” ns indicates that the mean silique lengths are not significantly different from unpollinated pistils harvested at 7 DPA.
Mean silique length of cross-pollinated gai siliques (gai self-pollinated siliques attained 8.0 ± 1.7 mm).
Siliques less than 2-fold the length difference between anthesis and unpollinated 7-d pistils.