Fig. 3.

FLT3-ITD⁺ cells are most susceptible to AC-4–130. a Viability assay for hematopoietic or control cell lines with AC-4–130 or DMSO (Ctrl) for 72 h. IC₅₀ values (µM) were determined using GraphPad Prism 5 software (GraphPad Software, Inc.). b MV4–11 and MOLM-13 cells were treated with AC-4–130 or DMSO (Ctrl) in a dose-dependent manner for 72 h or with 5 µM AC-4–130 in a time-dependent manner. Apoptotic cells were detected by AnnexinV/PI staining. Representative dot plots are shown. c Cell cycle distribution was determined after 72 h using PI staining