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. 2018 May 8;8:7182. doi: 10.1038/s41598-018-25208-y

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Cellular response to brain-implanted devices at multiple time points using PrismPlus mouse line. (a–c) Confocal images (merged) of cellular response to brain implanted devices at 1 week (a), 2 weeks (b) and 4 weeks (c) post implant. Dashed white ellipse represents the implant location. Merged images are separated into individual channels. Images are maximum projected intensity of 20 um thick sections and are approximately from the same depth (approximately 500 µm deep) in the cortex. Images in Fig. 4(a–c) were captured and processed using same settings. Objective: Plan-Apochromat 20x/0.8; Laser power: 405 nm: 5%, 488 nm: 6% and 543 nm: 0.2%. Scale bar = 100 µm.