Table 2.

MALDI-TOF MS results of 75 Tsukamurella species and 14 species of closely related genera using Bruker database and extended in-house database

Bacterial species	Total no. of isolates (n = 89)	No. of isolates resulting in the indicated score attained using:
		Bruker Reference Library V.6.0.0.0				Extended in-house database
		≥2.0	1.7–1.9	<1.7	Incorrect species identification	≥2.0	1.7–1.9	<1.7	Incorrect species identification
Type and reference (n = 15)a
T. paurometabola	1	1	0	0	0	1	0	0	0
T. inchonensis	1	1	0	0	0	1	0	0	0
T. strandjordii	1	0	0	1	1	1	0	0	0
T. pulmonis	2	0	1	1	2	2	0	0	0
T. tyrosinosolvens	2	2	0	0	2	2	0	0	0
T. pseudospumae	2	0	0	2	2	2	0	0	0
T. spumae	1	0	1	0	1	1	0	0	0
T. soli	1	0	0	1	1	1	0	0	0
T. serpentis	1	0	0	1	1	1	0	0	0
T. hongkongensis	1	0	0	1	1	1	0	0	0
T. serpentis	2	0	0	2	2	2	0	0	0
Clinical or veterinary (n = 74)
T. tyrosinosolvens	31	20	10	1	31	31	0	0	0
T. pulmonis	25	0	9	16	25	25	0	0	0
T. hongkongensis	2	0	0	2	2	1	1	0	1
T. sinensis	1	0	0	1	1	1	0	0	0
T. strandjordii	1	0	0	1	1	1	0	0	0
N. nova	3	3	0	0	0	3	0	0	0
N. cyriacigeorgica	2	1	1	0	0	1	1	0	0
N. brasiliensis	1	0	1	0	0	0	1	0	0
N. farcinica	2	1	1	0	0	1	1	0	0
R. equi	2	2	0	0	0	2	0	0	0
R. erythropolis	1	1	0	0	0	1	0	0	0
G. sputi	2	2	0	0	0	2	0	0	0
G. bronchialis	1	1	0	0	0	1	0	0	0

^a Among the 15 type and reference strains, only 6 (40.0%) showed the correct genus with score ≥1.7 by MALDI-TOF MS using the Bruker Reference Library V.6.0.0.0