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. 2017 Dec 20;31(2):171–191. doi: 10.1007/s00497-017-0320-3

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© The Author(s) 2017

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

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Fig. 2 — Arabidopsis floral organ lengths change in a position-dependent manner across flower positions 1–10. Floral phenotypes of wild type (Col-0) and ga20ox mutants, as specified, at flower positions one (a–h) and ten (i–p) under control growth conditions. All flowers shown are newly opened (floral stage 13; Smyth et al. 1990). Statistically significant three-way interactions were found between genotype, flower position and GA treatment for relative stamen length (p < 0.001) and absolute lengths of pistils (p < 0.001), stamens (p < 0.001) and petals (p = 0.002) across the early inflorescence. For sepals, a three-way interaction was not statistically significant (p = 0.275), but three separate significant two-way interactions were identified (genotype by GA, p < 0.001; genotype by flower position, p = 0.018; GA by flower position, p < 0.001). Mean floral organ lengths of newly opened (stage 13) flowers across flower positions 1–10 and position 15, showing stamen length as a percentage of pistil length (q) and absolute lengths (in mm) of pistils (r), stamens (s), petals (t). Flower position 15 is included as an indicator of floral organ growth in later flowering. Values shown are the mean of four independent flowers ± S.E. Genotypes not represented on individual graphs were not significantly different from wild type at any flower position (p > 0.05). Significant differences (p < 0.05) from wild type within each flower position are denoted by asterisks. Mean sepal lengths of stage 13 flowers, for each genotype under control growth conditions (black) and exogenous GA treatment (white) averaged across all flower positions measured (u) and for each flower position under control growth conditions (black) and exogenous GA treatment (white), averaged across all genotypes (v). Values shown are the mean of 44 (u) and 32 independent flowers (v) ± S.E. Letters denote significant difference (p < 0.01) from wild type under control growth conditions (black) or GA treatment (grey). Genotypes marked with different letters are significantly different from each other. Asterisks denote a significant effect of GA treatment within a genotype (u) or flower position (v). Pairwise comparisons in (q–v) were made using LSDs at a significance threshold of 5%, with pistil and stamen lengths being analysed on the square-root scale (see Online Resource 2)