Fig. 5.
Early floral developmental events are significantly affected by both GA signalling and flower position. The incidence of floral abnormalities was analysed by GLM (see “Materials and methods”). Statistically significant two-way interactions were detected between genotype and GA treatment (p = 0.024), genotype and flower position (p = 0.033) and between GA treatment and flower position (p < 0.001). There was no significant three-way interaction (p = 0.807). a Mean frequencies of floral abnormalities (collective deviations in expected floral organ number, organ fusion and organ homeosis) in ga20ox mutants averaged across the early inflorescence (flowers 1–10), under control (black) and GA-treated conditions (white). Values shown are the mean of 44 independent flowers ± S.E. Asterisks indicate a significant difference between control and GA-treated conditions (p < 0.05) within a genotype. Letters indicate significant difference (p < 0.05) of genotypes compared to wild type under either control growth conditions (black letters) or GA treatment (grey letters). Genotypes or flower positions denoted by different letters are significantly different from one another (p < 0.05). b Plotted mean frequencies of floral abnormalities at flower positions 1–10 and 15 under control growth conditions, comparing wild type to the ga20ox1 and ga20ox1 ga20ox3 mutants which showed significant differences in (a). Values shown are the mean of four independent flowers ± S.E. c Mean frequencies of floral abnormalities at flower positions 1–10 and 15 averaged across all genotypes, under control (black) and GA-treated conditions (white). Values shown are the mean of 32 independent flowers ± S.E. Asterisks indicate a significant difference between control and GA-treated conditions (p < 0.05) within a single flower position. Letters indicate significant difference (p < 0.05) of flower positions compared to position one under either control growth conditions (black letters) or GA treatment (grey letters). Flower positions denoted by different letters are significantly different from one another (p < 0.05) within the same growth condition. No comparison was made between different flower positions from separate growth conditions. Pairwise comparisons in (a, c) were made using LSDs with a significance threshold of 5% (see Online Resource 3)
