Figure 5.

Extracted fragment ATDs of Hex₅ and Hex₆-containing ions. (a) Ions of composition Hex₅-O-CH=CH-O^- (m/z 869). Red trace, ion ak from Man₅GlcNAc₂ (2). Blue and green traces, ions ag and ah from Man₆GlcNAc₂ (3) and Man₇GlcNAc₂ (4). Light blue trace, ion af from Man₉GlcNAc₂ (9). Purple trace from the d1d1d2 isomer of Man₈GlcNAc₂ (7), major peak, ion ai. Black trace, ion aj from glycans containing mannose 8 in the 6-antenna. Orange trace, ion al from glycans 13, 14, and 15, containing three glucose residues in the 3-antenna. (b) Fragments of composition Hex₆GlcNAc₁-O-CH=CH-O^- (m/z 1234). Red trace: ion am from Man₆GlcNAc₂. Blue trace, second peak, ion ao from the d1d1d3 isomer of Man₈GlcNAc₂ (6). Pink trace, ATD from Glc₃Man₈GlcNAc₂ (14) containing ions am and ao. Green trace, ion an from Man₉GlcNAc₂ (9) and its glucosylated analogues (12 and 15). (c) Fragments of composition Hex₆-O-CH=CH-O^- (m/z 1031) with structures similar to those in Panel a. Orange trace, ion aq from S. cerevisiae