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. 2018 May 8;8:7192. doi: 10.1038/s41598-018-25542-1

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Touch-induced calcium responses in RnA neurons. (a) Micrograph of the male tail showing the expression of tba-9::GCaMP5.0 in the rays. (b) A schematic illustrating of delivering mechanical stimulation toward the RnA cilia (at the position of rays 4–6 is shown). (c,d) Representative time-lapse rainbow images of GCaMP5.0 based calcium responses (c) and soma fluorescence changes (d) from R5A and R6A neurons induced by mechanical stimulation of 20 μm displacement at the position of ray5 and ray6.