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. 2018 May 2;9:934. doi: 10.3389/fimmu.2018.00934

Figure 7.

Figure 7

Cell-mediated immune response after prime-boost vaccination was enhanced in chitosan nanoparticles (CNPs)-KAg-vaccinated pigs at pre-challenge days post-vaccination (DPV) 35/day post-challenge (DPC) 0. Peripheral blood mononuclear cells (PBMCs) isolated from blood were stimulated with different variant influenza A virus (IAVs). IFNγ secretion in the culture supernatant and antigen-specific lymphocyte proliferation was determined after 72 h of stimulation with (A,E) H1N2-OH10, (B,F) H1N1-OH7, and (C,G) H3N2-OH4 IAVs. (D) Flow cytometry analysis of PBMCs showed enhanced frequency of CTLs (CD3+CD4−CD8αβ+) in CNPs-KAg-vaccinated pigs. Data represent the mean value of three to five pigs ± SEM. Statistical analysis was carried out using Kruskal–Wallis test followed by Dunn’s post hoc test. Asterisk refers to the statistical significant difference between the indicated two pig groups (*p < 0.05).