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. 2018 May 2;9:934. doi: 10.3389/fimmu.2018.00934

Figure 9.

Figure 9

Cell-mediated immune response in TBLN-MNCs of pigs vaccinated with chitosan nanoparticles (CNPs)-KAg at day post-challenge (DPC) 6. TBLN-MNCs isolated on the day of necropsy were stimulated with different variant swine influenza A viruses (SwIAVs), and secreted IFNγ into the culture supernatant was measured by cytokine ELISA against (A) H1N2-OH10, (B) H1N1-OH7, and (C) H3N2-OH4 influenza A virus (IAVs). (D) The frequency of T helper (Th)/memory cells (CD3+CD4−CD8α+) in TBLN-MNCs of CNPs-KAg-vaccinated pigs was analyzed by flow cytometry. The expression of Th1 (E) and Th2 (F) transcription factors were also determined in TBLN at DPC 6. Data represent the mean value of three to five pigs ± SEM. Statistical analysis was carried out using Kruskal–Wallis test followed by Dunn’s post hoc test. Asterisk refers to the statistical significant difference between the indicated two pig groups (*p < 0.05).