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. 2018 May 8;8:7239. doi: 10.1038/s41598-018-25718-9

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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3D cellular organization of D1 ORL UVA cells under microgravity. (a) Micrographs of cells levitated and assembled for 24 and 48 h (with 100 mM Gd-BT-DO3A) at different cell numbers (total 5000, 50000 and 500000 cells). Scale bars: 500 μm. (b–f) Quantitative description of the cellular clusters formed for 24 or 48 h with magnetic levitation (100 mM Gd-BT-DO3A) at different cell numbers (total 5000, 50000 and 500000 cells); (b) area, (c) perimeter, (d) elongation, (e) thickness and (f) length, and (g) position of the clusters between magnets (the top point of the bottom magnet:0, the bottom point of the top magnet: 1). Data are plotted as mean of replicates with error bars (±SD) and statistically analyzed using a two-way ANOVA and Sidak posthoc test. Statistical significance was defined as P < 0.05.