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. 2018 May 8;8:7188. doi: 10.1038/s41598-018-24056-0

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Post-UTI chronic pain is associated with MCP1 induction. MCP-RFP/CCR2-GFP mice were instilled with PBS or infected with E. coli strain NU14, SΦ874 or SΦ874/pDgal. Isolated sacral DRGs of saline-instilled mice have low RFP and GFP expression. GFP expression was marked in DRG cell bodies of mice infected with either NU14 or SΦ874/pDgal (arrowheads). DRGs of SΦ874-infected mice expressed RFP in fibers (arrow) colocalized with neuronal cell bodies expressing GFP (yellow, arrowhead).