Figure 7.

Genistein and PP2 route BmCPV to the wrong destination- lysosomes. (a) Normal BmN cells (control) were incubated with A546-labeled BmCPV virions and a lysosomal staining agent for 3 h; (b) BmN cells were treated with PP2 (0.16 μM) for 30 min, followed by incubation with A546-labeled BmCPV virions and a lysosomal staining agent for 3 h; (c) BmN cells were treated with genistein (50 μg/mL) for 1 h, followed by incubation with A546-labeled BmCPV virions and a lysosomal staining agent for 3 h; (d) quantification of the spectral overlap of BmCPV particles with lysosomes. The spectral overlap is presented as the percent co-localization (n = 21 cells). Error bars indicate standard deviations. ***P < 0.001.