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. 2018 May 8;8:7140. doi: 10.1038/s41598-018-25157-6

Figure 5.

Figure 5

The interplay between the S2 and S3 sites maximize CjFur DNA binding activity. (A) Schematic representation of apo-CjFur and holo-CjFur regulation. Key residues important for the gene regulatory activity of CjFur are listed and residues highlighted in red represent residues involved in both apo and holo regulation. Yellow, red and green circles represent S1, S2 and S3 metal binding sites, respectively. (B) Schematic representation of different metalation states of CjFur presented in this study. Filled and empty circles indicate metalated and unoccupied metal binding sites, respectively. The red X represent mutated metal binding sites. Mutations of S2 and S3 metal sites result in loss of metal binding in both S2 and S3 sites, decreased DNA binding activity in vitro and in C. jejuni and decreased ability to colonize chick ceca.