Table 1.
Summary of the residues important for DNA binding in apo-CjFur and holo-CjFur.
Regulatory metal ion | Mutations tested | Key residues | |
---|---|---|---|
Apo-CjFur EMSA | N.A. | R14E, K17E, R20E, K25E, K28E, R30E, Y68A, R69E | R20, K25, K28, R69 |
Apo-CjFur RT-qPCR (activation) | N.A. | R20E, K25E, R30E, Y68A, R69E | K25, R30, Y68 |
Apo-CjFur RT-qPCR (repression) | N.A. | R20E, K25E, R30E, Y68A, R69E | R20, K25, R30, Y68, R69 |
Holo-CjFur EMSA | Mn | R20E, K25E, K28E, R30E, Y68A, R69E | K25, R30, Y68, R69 |
Holo-CjFur RT-qPCR (repression) | Fe | R20E, K25E, R30E, Y68A, R69E | R20, K25, R30, Y68, R69 |
N.A. Not applicable.